Sunday, February 03, 2008
A New Method for Antimicrobial Susceptibility Testing of In Vitro-cultured Bacteria By Means of Resonance Light Scattering Technique.
Shi YJ, Chen J, Xu M.
School of Chemistry and Chemical Engineering, Nantong University, Nantong, Jiangsu 226000, P.R. China.
A new method for antimicrobial susceptibility testing of in vitro-cultured bacteria on an ordinary fluorescence spectrometer was developed. The viable bacteria reduced 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) to produce insoluble particles that displayed intense resonance scattering light (RSL). The assay showed a linear relationship between the number of viable bacteria and the intensity of resonance scattering light. Dead bacteria were unable to reduce MTT. Methicillin-resistant Staphylococcus aureus exposed to flavonoids from Marchantia convoluta showed a flavonoids concentration-dependent inhibition of the ability to reduce MTT. In the assay, less than 12 h were required to attain susceptibility results and fewer bacteria were utilized than in traditional methods.
The RLS technique could, in combination with the MTT assay, be a rapid and sensitive measuring method to determine the in vitro activity of new antimicrobials.
PMID: 18239427 [PubMed - in process]