Sunday, February 24, 2013
Candida glabrata Prosthetic Hip Infection.
Candida glabrata Prosthetic Hip Infection.
2012
Bartalesi F, Fallani S, Salomoni E, Marcucci M, Meli M, Pecile P, Cassetta MI, Latella L, Bartoloni A, Novelli A.
Source
Consultant Physician, Infectious and Tropical Diseases Department, Azienda Ospedaliero-Universitaria Careggi, Florence, Italy. bartalesif@aou-careggi.toscana.it.
Abstract
We present a case of a 60-year-old Caucasian woman carrying a 2-year-old hip prosthesis infected by Candida glabrata dose-dependent susceptible to fluconazole and voriconazole. Resection arthroplasty was performed. Six weeks of caspofungin plus liposomal amphotericin combination therapy achieved joint sterilization and allowed a successfully reimplantation arthroplasty. In addition we review 9 cases of C. glabrata prosthetic joint infection described to date in the literature.
Labels: arthroplasty, Candida glabrata, caspofungin, Infection, liposomal amphotericin, Prosthetic Hip
# posted by Pat O'Connor @ 8:32 AM
Wednesday, February 13, 2013
Evaluation of diagnostic value of procalcitonin as a marker of neonatal bacterial infections.
Evaluation of diagnostic value of procalcitonin as a marker of neonatal bacterial infections.
2012
Source
Pathology department, Hamadan University of Medical Sciences, Hamadan, Iran.
Abstract
KEYWORDS: Infection, Newborn, Predictive Value of Tests, Procalcitonin, Sensitivity, Specificity
OBJECTIVE:
This study tried to assess sensitivity, specificity, positive and negative predictive value of procalcitonin for diagnosis of neonatal bacterial infections.
METHODS:
This prospective cross sectional study was carried out during an 18-month period in NICU and neonatal wards of Besat Hospital in Hamedan province, Iran. 39 symptomatic infants with clinical and laboratory findings in favor of bacterialinfection with a positive blood, CSF, and/or supra pubic urine culture entered the study; 32 newborns without any bacterialinfection served as control group. Quantitative procalcitonin level ≥0.5 ng/ml was accepted as pathological. Finally sensitivity, specificity, positive (PPV) and negative predictive value (NPV) were calculated for procalcitonin test.
FINDINGS:
20 blood cultures, 17 urine cultures and 8 CSF cultures were positive. Sensitivity, specificity, PPV and NPV for procalcitonin test was 76.9%, 100%, 100% and 78% respectively. Diagnostic value of procalcitonin test in accordance with blood culture for mentioned items was 85%, 100%, 100% and 91.4% respectively. Its diagnostic value according to urine culture was: sensitivity 70.6%, specificity 100%, PPV 100% and NPV 86.4%, and according to CSF culture was: sensitivity 75%, specificity 100%, PPV 100% and NPV 94.1% respectively.
CONCLUSION:
The results show that the procalcitonin test has high sensitivity, specificity, PPV and NPV for diagnosis of neonatal infections.
Labels: blood cultures, Infection, newborn, Predictive Value of Tests, Procalcitonin, Sensitivity, Specificity, urine cultures
# posted by Pat O'Connor @ 7:57 AM
Role of Bacterial Lipopolysaccharide in Enhancing Host Immune Response to Candida albicans.
Role of Bacterial Lipopolysaccharide in Enhancing Host Immune Response to Candida albicans.
2013
Source
Tissue Engineering and Reparative Dentistry, School of Dentistry, College of Biomedical and Life Sciences, Cardiff University, Heath Park, Cardiff CF14 4XY, UK.
Abstract
Human infections involving yeast of the genus Candida often occur in the presence of bacteria, and, as such, it is important to understand how these bacteria influence innate host immunity towards Candida. Dectin-1 is a cell receptor of macrophages for Candida albicans recognition. The aim of this study was to examine dectin-1 expression by monocytes after stimulation with bacterial lipopolysaccharide (LPS), followed by heat-killed C. albicans (HKC). Freshly isolated human peripheral blood monocytes (PBMCs) and human monocytes cell line (THP-1) cells expressed low levels of dectin-1. Stimulation with LPS and GM-CSF/IL-4 was found to increase dectin-1 expression in both CD14(+) human PBMC and THP-1 cells. Enhanced dectin-1 expression resulted in increased phagocytosis of Candida. When THP-1 cells were challenged only with HKC, detectable levels of IL-23 were not evident. However, challenge by LPS followed by varying concentrations of HKC resulted in increased IL-23 expression by THP-1 cells in HKC dose-dependent manner. Increased expression of IL-17 by PBMC also occurred after stimulation with Candida and LPS. In conclusion, bacterial LPS induces an enhanced immune response to Candida by immune cells, and this occurs through increasing dectin-1 expression.
Labels: Bacterial Lipopolysaccharide, Candida albicans, Dectin-1, Host Immune Response, human monocytes cell line, peripheral blood monocytes
# posted by Pat O'Connor @ 7:26 AM
Thursday, February 07, 2013
A case of mycoplasma hominis infection on chronic refractory lower leg ulceration caused by livedo vasculopathy
A case of mycoplasma hominis infection on chronic refractory lower leg ulceration caused by livedo vasculopathy
2012
[Article in Japanese]
Source
Clinical Laboratory Department, St. Luke's International Hospital, Chuo-ku, Tokyo 104-8560, Japan. yamaji@luke.or.jp
Abstract
Mycoplasma hominis is a common inhabitant of the human urogenital tract and most frequently causes diseases of the genitourinary tract. Extragenital M. hominis infections are uncommon, with almost all occurring in immunosuppressed persons or those predisposed due to surgery or trauma. We report a case of non surgical, non-traumatic wound infectioncaused by M. hominis. A 28-year-old immunocompetent woman with livedo vasculopathy had an open wound on dorsum of her right foot with signs and symptoms of infection. However, gram staining of the wound swab demonstrated no microorganisms, and initial bacterial cultures did not reveal any microbial growth. After 2 days of culture, minute translucent colonies were appeared and subsequently identified as M. hominis. She was successfully treated with levofloxacin(LVFX). For the patient's being immune-competent, this infection seems to need a substantial bacterial transfer from the inhabitant organ. The transfer is likely mediated by the fluid's drop, for anatomical locations of vagina and the infection site on leg. Namely, the hinder leg infection is suspected to be caused by continual and heavy bacterial exposure originated from the vaginal M. hominis. This clinical case suggests that infections may occur even in normal immunological status if the site is close to, and lacks anatomical barrier from, the M. hominis inhabitant organ. Especially in infection at chronic refractory lower leg ulceration, M. hominis should be considered as a causative organism.
Labels: human urogenital tract, immunocompetent, Infection, levofloxacin, livedo vasculopathy, lower leg ulceration, LVFX, mycoplasma hominis
# posted by Pat O'Connor @ 7:01 AM
Carbon Catabolite Control in Candida albicans: New Wrinkles in Metabolism.
Carbon Catabolite Control in Candida albicans: New Wrinkles in Metabolism.
Feb 2013
Source
Department of Microbiology and Molecular Genetics, University of Texas Health Science Center at Houston, Houston, Texas, USA.
Abstract
Most microorganisms maintain strict control of nutrient assimilation pathways to ensure that they preferentially use compounds that generate the most energy or are most efficiently catabolized. In doing so, they avoid potentially inefficient conflicts between parallel catabolic and metabolic pathways. The regulation of carbon source utilization in a wide array of bacterial and fungal species involves both transcriptional and posttranscriptional mechanisms, and while the details can vary significantly, carbon catabolite control is widely conserved. In many fungi, the posttranslational aspect (carbon catabolite inactivation [CCI]) involves the ubiquitin-mediated degradation of catabolic enzymes for poor carbon sources when a preferred one (glucose) becomes available.
A recent article presents evidence for a surprising exception to CCI in the fungal pathogen Candida albicans, an organism that makes use of gluconeogenic carbon sources during infection (D. Sandai, Z. Yin, L. Selway, D. Stead, J. Walker, M. D. Leach, I. Bohovych, I. V. Ene, S. Kastora, S. Budge, C. A. Munro, F. C. Odds, N. A. Gow, and A. J. Brown, mBio 3[6]:e00495-12). In vitro, addition of glucose to cells grown in a poor carbon source rapidly represses transcripts encoding gluconeogenic and glyoxylate cycle enzymes, such as phosphoenolpyruvate carboxykinase (Pck1p) and isocitrate lyase (Icl1p), in both C. albicans and Saccharomyces cerevisiae.
Yet, uniquely, the C. albicans proteins persist, permitting parallel assimilation of multiple carbon sources, likely because they lack consensus ubiquitination sites found in the yeast homologs. Indeed, the yeast proteins are rapidly degraded when expressed in C. albicans, indicating a conservation of the machinery needed for CCI. How this surprising metabolic twist contributes to fungal commensalism or pathogenesis remains an open question.
Labels: Candida albicans, Carbon Catabolite, catabolic enzymes, glyoxylate cycle enzymes, Metabolism
# posted by Pat O'Connor @ 6:58 AM
